Differentiation Between Natural and Commercial Bee Venoms Using Fluorescence Spectroscopy
Honey bee venom (HBV, A. m. scutellata) contains polypeptides [melittin, mast cell degranulating peptide (MCD), adolapin and apamin] and enzymes.The aim of the present study was to differentiate between commercial and natural bee venoms using aqueous olive leaf extract (AOLE) employing fluorescence techniques. Tryptophan mainly responsible for the fluorescence and hence widely used as a tool to monitor conformational changes in proteins. Changes in the fluorescence intensities with blue shifts were obtained with bee venoms. Binding of AOLE constituents near the active site of the enzyme could be evidenced and possible modes of interaction are discussed. The fluorescence method used was rapid and sensitive and was able to differentiate between commercial and natural bee venoms utilizing AOLE.
(1) Zolfagharian H, Mohajeri M, Babaie M. Honey Bee Venom (Apis mellifera) Contains Anticoagulation Factors and Increases the Blood-clotting Time. J Pharmacopuncture 2015; 18(4):7-11.
(2) Frick M, Fischer J, Helbling A, Rueff F, Wieczorek D, Ollert M, Pfutzner W, Muller S, Huss-Marp J, Dorn B, Biedermann T, Lidholm J, Ruecker G, Bantleon F, Miehe M, Spillner E, Jakob T. Predominant Api m 10 sensitization as risk factor for treatment failure in honey bee venom immunotherapy. J Allergy Clin Immunol 2016; 138(6):1663-1671.
(3) FISCHER FG, DORFEL H. [Honeybee venom. II. Amino acid composition of bee venom fractions]. Biochem Z 1953; 324(6):465-475.
(4) Danneels EL, Van Vaerenbergh M, Debyser G, Devreese B, de Graaf DC. Honeybee venom proteome profile of queens and winter bees as determined by a mass spectrometric approach. Toxins (Basel) 2015; 7(11):4468-4483.
(5) Son DJ, Lee JW, Lee YH, Song HS, Lee CK, Hong JT. Therapeutic application of anti-arthritis, pain-releasing, and anti-cancer effects of bee venom and its constituent compounds. Pharmacol Ther 2007; 115(2):246-270.
(6) Park HJ, Lee SH, Son DJ, Oh KW, Kim KH, Song HS, Kim GJ, Oh GT, Yoon DY, Hong JT. Antiarthritic effect of bee venom: inhibition of inflammation mediator generation by suppression of NF-kappaB through interaction with the p50 subunit. Arthritis Rheum 2004; 50(11):3504-3515.
(7) Kamceva T, Flemmig J, Damnjanovic B, Arnhold J, Mijatovic A, Petkovic M. Inhibitory effect of platinum and ruthenium bipyridyl complexes on porcine pancreatic phospholipase A2. Metallomics 2011; 3(10):1056-1063.
(8) Markwell MA, Haas SM, Bieber LL, Tolbert NE. A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. Anal Biochem 1978; 87(1):206-210.
(9) Kenoth R, Simanshu DK, Kamlekar RK, Pike HM, Molotkovsky JG, Benson LM, Bergen HR, III, Prendergast FG, Malinina L, Venyaminov SY, Patel DJ, Brown RE. Structural determination and tryptophan fluorescence of heterokaryon incompatibility C2 protein (HET-C2), a fungal glycolipid transfer protein (GLTP), provide novel insights into glycolipid specificity and membrane interaction by the GLTP fold. J Biol Chem 2010; 285(17):13066-13078.
(10) Poller L, Keown M, Shepherd SA, Shiach CR, Tabeart S. The effects of freeze drying and freeze drying additives on the prothrombin time and the international sensitivity index. J Clin Pathol 1999; 52(10):744-748.
(11) Hogg PJ, Jackson CM, Labanowski JK, Bock PE. Binding of fibrin monomer and heparin to thrombin in a ternary complex alters the environment of the thrombin catalytic site, reduces affinity for hirudin, and inhibits cleavage of fibrinogen. J Biol Chem 1996; 271(42):26088-26095.
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