An organic solvent-free and quick determining method for routine residue monitoring of tetracycline and 4-epi-tetracycline in cow’s milk
Keywords:
International Harmonized Analytical Method, Organic Solvent-Free, HPLC, Residue Monitoring, Tetracycline, 4-Epi-TetracyclineAbstract
Under organic solvent-free conditions, a quick, easy, small-scale sample preparation followed by an isocratic mobile phase HPLC for quantifying tetracycline (TC) and its 4-epimer, 4-epi-tetracycline (4eTC), in cow’s milk was described in this paper. The sample preparation could be made only homogenization using a handheld ultrasonic-homogenizer with deproteining aqueous solution followed by filtration with the disposable unit. For determination and identification of analyte, an Inertsil WP300 C4 and isocratic 100% aqueous mobile with photodiode array detector were used. The method validation data were well within the international analytical method acceptance criteria. The total time required for the analysis of one sample was less than 7 min. In all the process, no organic solvents were used. The present method may be proposed as an international harmonized analytical method for routine residue monitoring of TC and 4eTC in cow’s milk.
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References
Anastas PT, Warner JC (1998). Green Chemistry - Theory and Practice, Oxford University Press.
Cherlet M, Schelkens M, Croubels S (2003). Anal. Chim. Acta, 492(1-2), 199-213.
Codex alimentarius, (2001). CAC/GL 37-2001: Harmonized IUPAC Guidelines for the use of Recovery Information in Analytical Measurement.
Codex alimentarius, (2009). CAC/GL 71-2009: Guidelines for the Design and Implementation of National Regulatory Food Safety Assurance Programme Associated with the Use of Veterinary Drugs in Food-Producing Animals.
European Commission (2000). Consideration of maximum residue limits at steps 6 (7), CL 1999/13 GEN, Washington, D.C.
European Commission (2009). Commission Regulation (EU) No. 37/2010, 22 Dec.
FDA/CDER (1994). Reviewer Guide, Validation of Chromatographic Methods.
Gajda A, Posyniak A (2015). Bull. Vet. Ins. Pulawy, 59(3), 345-352.
Ishibashi M (2007). J. Food Hyg. Soc. Jpn., 38, J194-J195.
Malisch R, Bourgeois B, Lippold R (1992). Deust. Leben. –Rund., 88 (7), 205-216.
Ogawa M, J (1996). Food Hyg. Soc. Jpn., 37, J289-J290.
Reddy BS, Sudhakar Y, Rao YS et al (2017). Oriental J. Chem., 33(5), 2459-2469.
De Ruyck H, De Ridder H (2007). Rap. Comm. Mass Spec., 21(9), 1511-1520.
Singh SP, Pundhir A, Ghosh, S (2015). Indian J. Nat. Pro. Res., 6(4), 293-298.
Sigma-Aldrich (2003), Product Information, Product Number T 3383.
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