A Simple HPTLC Method for Simultaneous Estimation of Atenolol and Chlorthalidone in Pharmaceuticals

Authors

  • Anna Pratima G. Nikalje Y. B. Chavan College of Pharmacy, Dr. Rafiq Zakaria Campus, Rauza Bagh, Aurangabad, Maharashtra, India.
  • Ramesh Gadikar Dr. Babasaheb Ambedkar Marathwada University Aurangabad

Keywords:

Chlorthalidone, Atenolol, Validation, densitometry, High-performance thin-layer chromatography (HPTLC)

Abstract

A new simple, precise, and accurate, high-performance thin-layer chromatography method was developed for the simultaneous determination of Atenolol and Chlorthalidone in tablet dosage forms. In this method, TLC aluminum plates pre-coated with silica gel 60 F254used as the stationary phase. The mobile phase consisting of Methylene Chloride: methanol: Ammonia solution 25%, in the ratio of 8.8:1.3:0.1 v/v is used. The densitometry evaluation was done at wavelength 266 nm using Camag scanner III. The developed method shows a well resolution between two peaks Atenolol and Chlorthalidone with Rf values 0.115 (±0.03) and 0.458(±0.03), respectively. The proposed method was validated as per International Conference on Harmonization (ICH) guidelines for parameters such as specificity, precision, accuracy, linearity, and ruggedness and reported the values. The linear regression analysis shows that the correlation coefficient R2 value for Atenolol is 0.999 and 0.997, respectively. The mean percentage recovery values obtained for Atenolol is 101.4 and for Chlorthalidone is 98.4. The statistical analysis shows that this method is suitable for routine analysis for quantification of Atenolol and Chlorthalidone in single or combined dosage forms of formulations.

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Author Biography

Anna Pratima G. Nikalje, Y. B. Chavan College of Pharmacy, Dr. Rafiq Zakaria Campus, Rauza Bagh, Aurangabad, Maharashtra, India.

Department of Pharmaceutical Chemistry

References

Orange book listed data at https://www.accessdata.fda.gov.

https://www.accessdata.fda.gov/drugsatfda_docs/label/2012/018760s029lbl.pdf

https://www.drugs.com/ppa/atenolol-and-chlorthalidone.html

Kumar B, Patel R, Bhandari A et al. (2006), Spectrophotometric method for estimation of Atenolol in tablet dosage form. Asian J Chem, 2006; 18: 3049-3052.

Lalitha G, Salomi PR. (2009), Development of an analytical method and its validation for the analysis of Atenolol in tablet dosage form by UV-Spectrophotometry. Int J Pharm PharmSci 2009; 5:197-9.

Anelise W, Daniele C, Melo JD, Karin G. et al. (2007), Validation of UV Spectrophotometric and HPLC Methods for Quantitative Determination of Atenolol In Pharmaceutical Preparations. Latin American Journal of Pharmacy.2007; 26(5):765-770.

Naveen Kumar, Verma N, Singh O, Joshi N, G.S. Kanwar GS. (2010), Estimation of Atenolol by Reverse Phase High Performance Liquid Chromatography. E J Chem. 2010; 7(3):962-966.

Basavaiah K , Chandrashekar U.(2006),Determination of Atenolol in pharmaceuticals by high performance liquid chromatography and spectrophotometry. Bulg Chem Commun,2006; 38: 104-111

Ceresole R ,Moyano MA ,Pizzorno MT ,Segal AI. (2006), Validated reversed-phase HPLC method for the determination of Atenolol in the presence of its major degradation product. J LiqChromatogrRelat Technol, 2006 ;29: 3009-3019.

Bhaskar BL, Anilkumar S ,Anilkumar VL. (2011), A facile and rapid HPLC method for determination of Atenolol in Pharmaceutical formulations. Asian Journal of Applied Sciences, 2011, 4(3); 306-313.

Singh B, Patel DK, Ghosh SK (2009), Reversed-Phase High Performance Liquid Chromatographic Method For Determination of Chlorthalidone In Pharmaceutical Formulation. International Journal of Pharmacy and Pharmaceutical Sciences.2009; 1(2):24-29.

www.drugbank.ca/DB00310/Chlorthalidone

Sharma R , Khanna S, Mishra GP (2013), RP-HPLC Method for Simultaneous Estimation of Atenolol, Hydrochlorothiazide and Losarton in Tablet Dosage form. Chemical Sciences Transactions, 2013, 2(11); 51-56.

Claudio G, Anna T, Sergio C, Giovanni Z (1997) , Simultaneous determination of Atenolol and Chorthalidone in plasma by high performance liquid chromatography application to pharmacokinetic studies in man. J. Chrom. B: Dio.Sci. App. 1997; 698:187-194.

Pimple HC, Rane SS, Patil HD, Chaudhari RY, Patil VR (2017), Simultaneous spectrophotometric estimation of Atenolol and Chlorthalidone in tablet dosage form. J Pharm BioSci 2017; 5(1):6-9.

Charde MS , Welankiwar AS, Chakole RD (2014), Simultaneous estimation of Atenolol and Chlorthalidone in combine tablet dosage form by absorption ratio method using UV-Vis spectrophotometry. International Journal of Advances in Pharmaceuticals, 2014, 3(1); 19-27.

Nivedita G, Akiful HM, Prashanth Kumar K, Pradeep Kumar T, et al. (2012) Simultaneous Estimation of Atenolol and Chlorthalidone as Bulk and In Tablet Dosage Form Using UV- Spectrophotometry. Journal of Pharmacy and Biological Sciences 2012; 1(4): 20-23.

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Published

2020-06-13

How to Cite

Anna Pratima G. Nikalje, & Ramesh Gadikar. (2020). A Simple HPTLC Method for Simultaneous Estimation of Atenolol and Chlorthalidone in Pharmaceuticals. To Chemistry Journal, 6, 35-47. Retrieved from https://purkh.com/index.php/tochem/article/view/808

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Research Articles